Tracking Leukocytes in vivo

نویسندگان

  • Ahmed S. Mahdi
  • Hassan I. Mathkour
چکیده

Leukocyte tracking invivo is a state of the techniques used for understanding the complex nature of leukocyte recruitment and the transmigration through endothelial cells. It known that the governing force acting on the individual cells are mainly hydrodynamic, the magnitude and nature of the force is not precisely known. This work was a novel segmentation algorithms based on fuzzy logic to track a group of recruited leukocytes captured from video images obtained from invivo experiment on a rat venule. We have found that the force acting on the leukocyte is 0.013998746 dynes under the given experimental conditions. Keywordsleukocytes, invivo, hydrodynamic, governing.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Automatic tracking of rolling leukocytes in vivo.

The analysis of instantaneous and average rolling leukocyte velocity is crucial to the study of inflammatory disease. In order to record features associated with leukocyte rolling, the leukocyte position must be tracked, typically by manual observation. Automated tracking of leukocytes is possible for in vitro studies, but not for recordings resulting from intravital experiments. Therefore, we ...

متن کامل

Consumption of antioxidant dietary agents, curcumin and vitamin C, protects cellular DNA from gamma-radiation

Background: Exposure to ionizing radiation results in genotoxicity and the unrepaired lesions in cellular DNA results in cell cycle arrest, reproductive death, interphase death, division delay, chromosome aberrations, mutations, etc. leading to the intensive destruction of cells and violation of their proliferative capacity there by adversely affecting the mammalian system. Since ionizing radia...

متن کامل

TRACKING LEUKOCYTES FROM IN VZVO VIDEO MICROSCOPY USING l\rlORPHOLOGICAL ANISOTROPIC DIFFUSION

The study of inflammatory disease hinges upon the behavior and movement of leukocytes and their interaction with the endothelium. We put forth a method for tracking leukocytes in vivo, whereas tracking has been demonstrated previously only for in vitro experiments. The tracker is based on the enhancing capability of morphological anisotropic diffusion, a partial differential equation model for ...

متن کامل

SEQUESTRATION OF LEUKOCYTES BY THE ISOLATED PERFUSED RAT LUNG

The sequestration of rat leukocytes and bone marrow cells by the lung vasculature was studied using an isolated perfused rat lung preparation. The passage of latex particles of 7.6 µm in diameter and non-hematopoietic cells through the lung blood vessels was also studied. Leukocytes and bone marrow cells were reversibly sequestered from circulation, whereas, latex particles and non-hematop...

متن کامل

Recent advances in microscopic techniques for visualizing leukocytes in vivo

Leukocytes are inherently motile and interactive cells. Recent advances in intravital microscopy approaches have enabled a new vista of their behavior within intact tissues in real time. This brief review summarizes the developments enabling the tracking of immune responses in vivo.

متن کامل

Leukocyte arrest during cytokine-dependent inflammation in vivo.

Leukocyte rolling along the walls of inflamed venules precedes their adhesion during inflammation. Rolling leukocytes are thought to arrest by engaging beta2 integrins following cellular activation. In vitro studies suggest that chemoattractants may instantaneously activate and arrest rolling leukocytes. However, how leukocytes stop rolling and become adherent in inflamed venules in vivo has re...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2014